Femtosecond digital lensless holographic microscopy to image biological samples.
Identifieur interne : 000694 ( Main/Exploration ); précédent : 000693; suivant : 000695Femtosecond digital lensless holographic microscopy to image biological samples.
Auteurs : RBID : pubmed:23988914English descriptors
- KwdEn :
- MESH :
- chemical : Tin Compounds.
- instrumentation : Holography, Microscopy.
- methods : Holography, Microscopy.
- Animals, Drosophila melanogaster, Head, Image Processing, Computer-Assisted, Time Factors.
Abstract
The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.
PubMed: 23988914
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A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23988914</PMID><DateCreated><Year>2013</Year><Month>08</Month><Day>30</Day></DateCreated><DateCompleted><Year>2014</Year><Month>04</Month><Day>29</Day></DateCompleted><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1539-4794</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>17</Issue><PubDate><Year>2013</Year><Month>Sep</Month><Day>1</Day></PubDate></JournalIssue><Title>Optics letters</Title><ISOAbbreviation>Opt Lett</ISOAbbreviation></Journal><ArticleTitle>Femtosecond digital lensless holographic microscopy to image biological samples.</ArticleTitle><Pagination><MedlinePgn>3205-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1364/OL.38.003205</ELocationID><Abstract><AbstractText>The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mendoza-Yero</LastName><ForeName>Omel</ForeName><Initials>O</Initials><Affiliation>Institut de Noves Tecnologies de la Imatge (INIT), Universitat Jaume I, 12071 Castelló, Spain.</Affiliation></Author><Author ValidYN="Y"><LastName>Calabuig</LastName><ForeName>Alejandro</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Tajahuerce</LastName><ForeName>Enrique</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Lancis</LastName><ForeName>Jesús</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Andrés</LastName><ForeName>Pedro</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Garcia-Sucerquia</LastName><ForeName>Jorge</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Opt Lett</MedlineTA><NlmUniqueID>7708433</NlmUniqueID><ISSNLinking>0146-9592</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Drosophila melanogaster</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Head</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Holography</DescriptorName><QualifierName MajorTopicYN="N">instrumentation</QualifierName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Image Processing, Computer-Assisted</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy</DescriptorName><QualifierName MajorTopicYN="N">instrumentation</QualifierName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>8</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>8</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">260306</ArticleId><ArticleId IdType="pubmed">23988914</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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